Answer:
By way of introduction, A260/A280 ratio is use to measure Protein Contamination. This procedure was first described to measure protein purity in the presence of nucleic acids. However it is now commonly used to assess protein contamination of DNA. The method to determine the concentration of protein contamination by using A260/280 ratio method is well explained below by filling in the missing words.
Explanation:
The A260/A280 ratio method estimates protein __purity___ by measuring the absorbance maximum at 280nm caused by the amino acids__cytosine___, ____Adenine____, and ___Guanine_________ Since ___Spectrophotometer____ also absorbs in the UV range, we can correct for this contaminate by measuring the absorbance maximum at ____260nm______ and using the following equation: Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml___ When extrapolated from a standard curve, the Bradford data indicates the amount (in _ug__ ) of __unknown ___ protein found in a sample. If you know the volume of sample that was added to the assay then you can calculate the protein ____concentration ___ of the sample. The coomassie blue dye in the Bradford assay specifically binds primary ¬¬___sulfonic___ and __positive amines__ groups of the amino acid side groups of the proteins. The more the dye binds to the sample, the _anionic_ the blue color will be, and the absorbance at 595¬nm will be_shifted Amax___. A sample with an unusually __protein___ number of ___280nm of Tyrosine __ [give a specific example] amino acids will underestimate the total amount of protein present in the sample.
Xerostomia
The medical term for this condition is xerostomia. Xerostomia
refers to abnormal dryness of the mouth which may be caused by a reduction in
the volume of saliva. Saliva is an important fluid in the body that assists to
protect and maintain oral health.
A.......................................................
Ptolemy's model had Earth at the center, and the moon's revolved around the planets instead of the planets revolving around the moon and sun. The planets' orbits' moved instead of the planets as well. Geocentric has Earth at the center and heliocentric has the Sun at the center
Answer:
The correct answer will be amino acids.
Explanation:
The process of protein synthesis takes place in an organelle called ribosomes. The ribosome reads the information coded in the mRNA in the form of triplets of nitrogenous bases also known as "codons". The codons code for specific amino acids which are assembled or joined via peptide bonds in the ribosomes.
In the question, Katie wanted to make doghouse of the wood and nails in which if doghouse represents the protein, the nails and wood will represent the basic unit or monomer of a protein called amino acids which are put together by a hammer.
Thus, amino acids are the correct answer.
