Answer:
1) UUC-AUG-AUC-AAG-UAA.
2) 48
Explanation:
<u>1) Determine the possible antisense oligo nucleotides possible from this amino acid sequence</u>
Given that the peptide sequence is = Glu-Glu-His-Asp-Val-Leu 1.
we will use a codon table to show the codons and Anticodons for each of the Amino acids
Amino Acid Codons Anticodons
Glu GAA, GAG UUC, CUC
His CAU, CAC AUG, GUG
Asp GAU, GAC AUC, GUC
Val GUU, GUC, GUA, GUG AAG, GAC, UAC,CAC
Leu UUA, UUG, CUU, CUC, CUA, CUG | UAA, CAA, AAG, GAG,UAG,CAG
From the table above
The possible antisense oligo nucleotide sequence is : UUC-AUG-AUC-AAG-UAA.
<u>2) How many total unique different oligonucleotide would need to be produced to cover all possibilities</u>
A desired peptide sequence requires 16 anticodons
Hence the total unique different oligonucleotide needed to be produced to cover all possibilities = 16 *3 = 48
