Answer:
The correct order is dehydration, embed in wax, cut into sections, staining
Explanation:
There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.
These steps are:
- Obtention of the material: The tissue is cut to an adequate size.
- Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.
- Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
- Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.
- Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about 5 to 20 micrometers of thickness.
- Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.
Explanation:
correlation relationships are between variables, however,it does not automatically mean that the change in one variable is the cause of the change in the values of the other variable. Causation indicates that one event is the result of the occurrence of the other event there is a causal relationship between the two events.
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Tourism can affect the swamp water, if traveled on a boat, with all the oil and stuff that is needed to get the boat running, and over hunting, can slowly kill all the crocodiles that live there affecting the crocodile population! <span />
Glacial lakes are bodies of water that are influenced by the presence of glaciers. ... Glacial lakes are common features around the margins of glaciers and ice sheets, and often evolve from ice-contact lakes into ice-distal lakes as glaciers and ice sheets margins retreat and become spatially separated from the lake.
Answer:
The same number of particles in a gas spread further apart than in the liquid or solid states. The same mass takes up a bigger volume . This means the gas is less dense. Density also depends on the material.
