Answer:
The correct order is dehydration, embed in wax, cut into sections, staining
Explanation:
There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.
These steps are:
- Obtention of the material: The tissue is cut to an adequate size.
- Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.
- Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
- Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.
- Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about 5 to 20 micrometers of thickness.
- Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.
Answer:
I believe that the lizards from the two populations, both short legged and long legged will still mate and reproduce. First of all, the lizards are still of the same species. Secondly, the lizards would mate and reproduce because it will increase their species chances of survival. In general, any chance of increasing survival rates will be taken in any natural circumstance.
Explanation:
Answer:
Store and transfer genetic information
Explanation:
Answer:
The correct answer will be option-A and B.
Explanation:
Transcription is a process which synthesizes a complementary molecule of DNA which acts a messenger called RNA.
The gene contains a sequence of the nucleotide at the beginning of gene called promoter sequence. The promoter sequence has the ability to attach an RNA synthesizing enzyme called RNA polymerase.
The RNA polymerase is the enzyme which synthesizes the RNA molecules using a single strand of DNA called template strand. RNA polymerase binds nucleotide at 3' end of the strand thus proceeding the strand in 5' to 3' direction.
The promoter and RNA polymerase begins the process of transcription and thus option-A and B is the correct answer.