In electrophoresis, the molecules travel between the positive electrode and the negative electrode carried by the current that is generated between them.
As seen in the image below, short fragments, due to their smaller size can travel faster than the longer fragments, so the fragments that have traveled the furthermost are the shortest. (marked E in the image)
As seen in the image below, short fragments, due to their smaller size can travel faster than the longer fragments, so the fragments that have traveled the furthermost are the shortest. (marked E in the image)