Answer:
The answer would be A, only chloroplasts and vacuoles are found in plant cells. :)
Enzymes lower activation energy through various means, including positioning substrates together in the proper orientation, applying torque on the substrates, providing the proper charge or pH microenvironment, and adding or removing functional groups on the substrates.
Option <u>(c.) the purity of the protein</u>, will be the right one.
Sodium Dodecyl Sulphate provides a negative charge to each protein as a function of their molecular size. It is an anionic detergent, as well as it is there to determine the relative abundance of major proteins.
After denaturing, proteins get unfolded and gets coated with SDS detergent molecules. The SDS-PAGE technique is used for the separation of protein based on their molecular size/weight/mass.
It is so because as it identifies the proteins by its molecular weight. Therefore, SDS is used in denaturing polyacrylamide gel electrophoresis for the determination of protein molecular weight, the SDS-PAGE is a technique.
To learn more about SDS-PAGE here
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