Answer:
A. 
B. 
C. 
D. 
Explanation:
Hello.
In this case, we must compute the final concentration in all the cases so we solve for it in the given equation:

Thus, we proceed as follows:
A. Here, the final diluted solution includes the 300 μL of the 5 mg/ml-BSA and the 700 μL of TBS.

B. Here, the final diluted solution includes the 50 μL of the 1.5 mg/ml-BSA, the 450 μL of water and the 500 μL of TBS.

C. Here, the final diluted solution includes the 10 μL of the 1 mg/ml-BSA and the 990 μL of TBS.

D. Here, the final diluted solution includes the 10 μL of the 0.1 mg/ml-BSA and the 990 μL of TBS.

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Answer:
<u><em>This is because the enzymes might work best at higher temperature than 37 degrees Celsius. Increase in temperature will increase the catalysis of the reaction.</em></u>
Explanation:
<em>When the temperature is increased, the molecules possess greater kinetic energy. This will allow more substrate and enzymes to collide. As a result, there will be more enzymes taking place in the reaction which will cause the rate of the reaction to increase.</em>
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However, if the temperature goes too high, then the shape of the active site of the enzymes will get changed. The enzyme will deactivate and the reaction will stop.
It is the “parent” generation, or the “paternal” one which the rest are observed from.