Answer:
Gene knockout is a technique used to determine the function of a gene that has already been sequenced, which is achieved by analyzing the phenotype of the individual carrying the knockout mutation(s). Moreover, gene sequencing is a technique used to determine the sequence of a given gene, which allows to determine how gene variants (polymorphisms) may be associated with the phenotypes of the target trait.
Explanation:
In genetics, gene knockout is a technique used to trigger mutations in a (already) sequenced gene in order to inactive its function and observe the resulting phenotype for a particular trait. This approach that starts with the inactivation of a given gene and ends with the phenotype is known as reverse-genetics. On the other hand, gene sequencing can be defined as the methodologies/techniques/tools used to determine the nucleotide base pair sequence of a particular gene. The gene knockout technique involves knowing a priori the gene sequence in order to obtain a gene knockout (gene KO). The combination of the information obtained from these techniques can be used to determine how variation (genetic variation) affects the expression of a phenotypic trait.
Answer: Yes. Splicing can be done in different ways to yield different mRNAs wich will create different proteins. Prokaryotes are not able to do this.
Explanation:
DNA (deoxyribonucleic acid) is a molecule that contains the genetic information for synthesizing amino acids that form proteins. To do this, DNA must first be transcribed into RNA (ribonucleic acid) and this is the molecule used for protein synthesis (translation). The newly transcribed RNA (called primary messenger RNA) from DNA results in a very long molecule and also has regions that do not code for anything, called introns, which are removed by a process called splicing. Exons are segments in the RNA that do code for amino acids and remain in the mature mRNA after splicing.
<u>Splicing is a process by which introns are cleaved from the primary messenger RNA and exons are joined to generate mature messenger RNA.</u> In addition, alternative splicing occurs which allows different mRNA isoforms and thus different proteins to be obtained from a primary mRNA transcript. This is because the exons will be joined or spliced in different ways, giving rise to different mature messenger RNA sequences. This process occurs mainly in eukaryotes, although it can also be observed in viruses. But it does not take place in Prokaryotes (Bacteria).
In summary, exons/introns can be spliced together in different ways to yield different mRNAs sequences. Each different mRNA sequence will code for a different protein.
