1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
babunello [35]
1 year ago
13

If you subcultured a microbial colony by transferring some of the cells into a tube of sterile nutrient broth, would the new cul

ture still be considered a pure culture?
Social Studies
1 answer:
podryga [215]1 year ago
8 0

1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.  

2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.

3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.

3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.

3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.

3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.

Learn more about subcultured at

brainly.com/question/26525141

#SPJ4

You might be interested in
How might the application of purely democratic ideas to a functioning government cause a problem for a large federal state?
Mice21 [21]
A purely democratic government would entail a pure vote including all people, of which the decision is made based on the majority. There would be no balance in this situation to account for even a large amount of minority voters. 
6 0
3 years ago
Read 2 more answers
How is power shared with small units in the United Kingdom?
WITCHER [35]

Answer: The legislature is divided into two separate houses, the House of Commons and the House of Lords. While the House of Lords is appointed, and maintains very little actual legislative power, the House of Commons is elected, and is where actual sovereign power resides

Explanation:

<em>My explanation of this would be:</em>

<em>Basically the federalism inside the united kingdom is the distribution of the power being shared between the two countries but HOWEVER England is by far the largest single unit in the U.K with both the populations of "84%" And the area would be around 53%/54%  </em>

<em />

7 0
3 years ago
How were Van Buren's beliefs different from<br> George Washington's?
makvit [3.9K]

Answer:

When assessing the impact and legacy of Martin Van Buren, scholars have ... that his efforts were the work of a cynical, manipulative, and power-hungry politician. ... treasury system—reflected his Jeffersonian and Jacksonian political beliefs. ... principles, he would not choose (and saw no need to choose) another path.

Explanation:

5 0
3 years ago
In one study described in the textbook, infants with difficult temperaments were randomly assigned to either a condition in whic
marta [7]

Answer:

The answer is an experimental group.

Explanation:

An experimental group, also called a treatment group, consists of a group that usually receives some kind of treatment during an experiment. The group is usually composed of test subjects like people, animals, plants, among others. The treatment refers to the variable that is being studied.

When the group does not get the appropriate treatment is called the control group. There is one difference between the control group and the experimental group and it could be the hypothesis you are working with.      

4 0
3 years ago
Presently, does the U.S. have a surplus or a deficit budget?
irina [24]

Answer:

U.S. government - Budget surplus or deficit 2000-2025. In 2019, the U.S. government had a budget deficit of 0.53 trillion U.S. dollars. This is compared to 2000, when the government had a budget surplus of 0.24 trillion U.S. dollars.

Explanation:

i hope i helped =)

7 0
2 years ago
Other questions:
  • A rule of thumb for whether you can violate norms in a relationship is to not overexpend your ____________.
    10·1 answer
  • PLEASE HELP!! <br> Short answers would be appreciated! (:
    5·1 answer
  • Which statement best supports the idea that the United States has a progressive income tax?
    14·2 answers
  • True or False: Buddhism teaches that human suffering is caused by angry gods
    14·1 answer
  • Social studies multiple choice
    13·2 answers
  • If 1,000 people are on the Mayflower, and 45 people died, and 520 people were sick, how many people lived and got through the si
    6·2 answers
  • What was the main reason for the Federal goverment to relocate american Indians in the 1830?
    6·1 answer
  • PLEASE HELPPPP!!!
    14·2 answers
  • True or false: The capacity to explore one's identity during adolescence and emerging adulthood is linked to the quality of frie
    9·1 answer
  • Conflict is inevitable phenomenon of human society.Explain​
    6·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!