Question

The ________ plate count (SPC) method involves diluting 1.0m of bacterial culture into a series of water blanks, and then taking a sample from the water blanks to add to empty petri plates which will be filled with melted agar.

Answer 1

The standard plate count (SPC) method involves diluting 1.0m of bacterial culture into a series of water blanks, and then taking a sample from the water blanks to add to empty petri plates which will be filled with melted agar.

The standard plate count is a method used in microbiology, which is used to gain an insight to estimate the density of bacterial population which is present in a bacterial culture broth. This is done by plating a small concentration of the culture in a petri-dish and then counting the colonies which form in the petri-plate. This method is used mostly in the food industry, to find the density of mesophilic bacteria in food. This method is extremely essential to determine the primary source of the bacterial contaminant.

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