Question

To date, which of these has been used largely in creating clones? Metabolic profiling RNA sequencing Protein mapping Recombinant DNA technology

Answer 1

RNA sequencing technology has been used largely in creating clones.

What is RNA sequencing?

RNA sequencing (RNA-Seq) makes use of high-throughput sequencing techniques to shed light on a cell's transcriptome.

Numerous RNA biology topics, such as single-cell gene expression, translation (the translatome), and RNA structure, can be studied using RNA-seq techniques (the structurome). Exciting new uses, such as spatial transcriptomics, are being investigated (spatialomics).

The steps in a typical RNA-sequencing experiment are as follows:

1. Design experiment: Plan the experiment.
2. Preparation of RNA. Purify and isolate the input RNA.
3. Library preparation. Create cDNA from the RNA and add sequencing adapters.
4. Sequence. cDNAs should be sequenced using a platform.
5. Analysis.

Learn more about RNA sequencing here:

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