Question

In sanger sequencing, what causes dna synthesis to terminate at a specific base?.

Answer 1

Hydroxyl-free nucleotide triphosphates causes DNA synthesis to terminate at a specific base in sanger sequencing.

What is sanger sequencing?

• The "chain termination method," often known as Sanger sequencing, is a technique for figuring out the nucleotide sequence of DNA.

• The Sanger Sequence is a method that was created in 1977 by two-time Nobel laureate Frederick Sanger and his colleagues.

What is DNA synthesis?

• Deoxyribonucleic acid molecules are produced either naturally or artificially during the process of DNA synthesis.

• A macromolecule known as DNA is composed of repeated nucleotide units joined together by covalent and hydrogen interactions.
• DNA biosynthesis takes place during the replication process that occurs when a cell divides.
• The DNA double helix is separated, and then, using the parent DNA chain as a template, a complementary DNA strand is synthesized.

Learn more about DNA synthesis here:

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