Answer:
In recent years political factors in the form of political unrest (such as the Arab spring) and terror attacks have had an increasing impact on tourism demand (cf. ... We continue to increase how much we travel in the long term, but the demand for certain affected destinations decreases.
Explanation:
Cahokia was made with rapidly speed because the all people participated in the construction of Cahokia.
What did the Cahokians build in their city?
It was made by the the Mississippians, a group of Native Americans. In the 1100's, Cahokians built a two-mile stockade around their city, which was protected by the guard towers of about 70 feet each tower. The first was protected by double-walled. About three times over the centuries, it was rebuilt in single-walled fashion. Cahokia Mounds is a National Historic Landmark area which is designed site for state protection.
So we can conclude that: Cahokia was made with rapidly speed because the all people participated in the construction of Cahokia.
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D. A frame is a list of the individuals in the population being studied.
1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
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