Question

A three banded isoenzyme pattern with a 1:2:1 intensity ratio indicates a ________________ protein.

Answer 1

A three banded isoenzyme pattern with a 1:2:1 intensity ratio indicates a dimeric protein.

• Isozyme is a multimeric forms of enzyme having similar specificity of the substrate but differences in kinetics and molecular weights of the enzymes.
• The banding patterns of isozyme observed from electrophoresis is related to the genetic conditions in which different combination of polypeptide chains exhibited under the mendelian principles.
• Hence with the intensity of 1:2:1 ratio, three banded phenotypes are formed having two homomeric and one heteromeric form from the dimeric protein in both multiple alleles at single loci and multiple /single allele at multiple loci.

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