differentiate between these two steps, you add an unknown inhibitor to a suspension of rapidly respiring mitochondria. After sub

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differentiate between these two steps, you add an unknown inhibitor to a suspension of rapidly respiring mitochondria. After sub

jecting the suspension to MAGIC analysis, you find that there is an accumulation of reduced ubiquinone and that Complex III is oxidized.

Biology

1 answer:

Eva8 [605] · Answer 1 · 2023-09-17T14:14:52+03:00

On MAGIC analysis it is found that reduced ubiquinone is being accumulated and complex III is oxidized.

Is Complex III reduced or oxidized?

Process - During each two-cycle process, the modified Q cycle in Complex III reduces cytochrome c, oxidizes ubiquinol to ubiquinone, and transfers four protons into the intermembrane gap.

Does ubiquinone get oxidized or reduced?

At the cellular level, CoQ10 exists as a redox pair, or in two forms that alternate back .
Forth as CoQ10 transfers a hydrogen in the mitochondria's electron transfer chain: ubiquinone, which is the oxidized form, and ubiquinol, which is the reduced form.

What is the function of complex III?

The mitochondria of cells, which transform the energy from food into a form that cells can utilise, are where Complex III is found.
Cells obtain a great deal of their energy from a process called oxidative phosphorylation, which is carried out by a number of complexes, including Complex III.

Learn more about Complex III.

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The complete question is -

We now get to the crux of the matter. If we are to identify which step is blocked, we will need at least two specific pieces of information.

Now let's put it all together . If you were told that ubiquinone was reduced but cytochrome c was oxidized , you would know that the pathway was blocked somewhere between these two carriers , but you would not necessarily know whether the block was at the ubiquinone ⇒ Complex lll step or the Complex III ⇒ cytochrome c step.

To differentiate between these two steps, you add an unknown inhibitor to a suspension of rapidly respiring mitochondria. After subjecting the suspension to MAGIC analysis, you find that there is an accumulation of reduced ubiquinone and that Complex III is oxidized.