In the biosynthesis of scopoletin, 2-oxoglutarate-dependent dioxygenase is an essential enzyme.
Coumarins are obtained from plants. This is done by one of the pathways called are phenylpropanoid pathway. The structure of coumarins is 2H-1-benzopyran-2-one. For the production of coumarin one of the major steps is ortho-hydroxylation. In the biosynthesis of scopoletin, Arabidopsis thalianais also plays a vital role in accumulating in its roots scopoletin and its beta-glucopyranoside scopoletin. When mutants of caffeoyl CoA O-methyltransferase 1, T DNA was inserted it was observed in the roots that there was a significant reduction in the amount of scopoletin and scopoletin.
It also showed the activity of recombinant CCoAOMT1 exhibited 3'-O-methyltransferase on caffeoyl CoA and resulting in feruloyl CoA. This led to the suggestion that one of the important precursors is feruloyl CoA in scopoletin biosynthesis. When T-DNA insertion mutants of F6'H1 were inserted in A. thaliana. Reductions in the levels of scopoletin and scopoletin in the roots were observed.
So the F6'H1 protein recombination demonstrated ortho-hydroxylase action for feruloyl CoA to generate 6'-hydroxyferuloyl CoA.
Therefore, 2-oxoglutarate-dependent dioxygenase is an essential enzyme in the biosynthesis of scopoletin.
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