Question

DNA contains the template needed to copy itself, but it has no catalytic activity in cells. What catalyzes the formation of phosphodiester bonds between adjacent nucleotides in the DNA polymer being formed during DNA replication? (A) ribozymes (B) DNA polymerase (C) ATP (D) RNA primers

Answer 1

DNA contains the template needed to copy itself, but it has no catalytic activity in cells. DNA polymerase (Option B) catalyzes the formation of phosphodiester bonds between adjacent nucleotides in the DNA polymer being formed during DNA replication.

DNA polymerase catalyzes the formation of the phosphodiester bond by adding the deoxynucleoside triphosphate onto the growing polynucleotide chain. During this step-wise process, a single pyrophosphate molecule is released every time the chain is elongated by one deoxyribonucleotide.

The main function of the third polymerase is duplication of chromosomal DNA, while others are involved mostly in DNA repair and translation.

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