Question

A scientist wants to begin making a DNA fingerprint, but she has only a very small DNA sample. What should her next step be

Answer 1

For obtaining an adequate amount of DNA samples for DNA fingerprinting, polymerase chain reaction (PCR) can be used.

Polymerase chain reaction (PCR) is a technique that is used to amplify the DNA sample. The steps included in PCR are:-

1. Denaturation of the double-stranded DNA (at around 95°C)

2. Annealing of the primers to each of the single-stranded DNA templates ( at around 65°C)

3. Extension or elongation of the primers by considering every single-stranded DNA as a template. This elongation is carried out by Taq polymerase (at around 72°C).

With each cycle of PCR, the DNA sample is increased by 2ⁿ. Here, 'n' represents the number of PCR cycles.

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Learn more about polymerase chain reaction here: -

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