Question

Calculate the percentage ionization in these amino acid side chains at the ph values specified:

Answer 1

The Henderson-Hasselbalch equation and pKa can be used to determine the % ionization if it is assumed that the amino and carboxyl groups are not preventing ionization.

1. aspartate at ph 5.4 = 98.3%
2. glutamate at ph 11.8 = 100%
3. histidine at ph 8.0 = 99%

Henderson-Hasselbalch equation- Henderson-Hasselbalch Equation to determine the pH of a solution. Knowing the pH of a solution is particularly significant for many chemical processes as well as for biological systems. The estimated pH value of a buffer solution may be determined using the Henderson-Hasselbalch equation.

pH- A solution's acidity or alkalinity may be determined based on the concentration of hydrogen ions in the solution, or pH. Typically, the pH scale runs from 0 to 14.

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