A monohybrid cross is made up of two organisms that have distinct variants at the same genetic locus. In a monohybrid cross, the character(s) under investigation are controlled by two or more mutations at a single gene locus. Each parent is selected to be homozygous or true breeding for a particular characteristic in order to perform such a cross (locus). When a cross meets the requirements to be classified as a monohybrid cross, it is typically identified by a distinctive distribution of second-generation (F2) offspring, also known as the monohybrid ratio. A monohybrid cross is a cross between two homozygous individuals, which produces the opposite phenotype for a particular genetic characteristic.
