Question

how many dna molecules would there be after four rounds of pcr if the initial reaction mixture contained two molecules?

Answer 1

1. Answer: 32 molecules of DNA would be after four PCR if the initial reaction mixture contained two molecules

• After 'n' rounds of the polymerase chain reaction, it is feasible to produce 2 n DNA molecules from a single DNA template molecule (PCR). Therefore, 2 to the power 4  = 16 DNA molecules will be produced after 4 PCR cycles
• If two molecules are doubled four times, 32 DNA molecules are created.

Explanation:

The PCR method is what?

The highly sensitive PCR method enables quick DNA amplification of a particular section. Using visual methods based on size and charge, PCR may detect and identify gene sequences by producing billions of copies of a certain DNA fragment or gene.

What does a PCR have to accomplish?

A given DNA segment can be quickly multiplied (amplified) into millions or billions of copies using the polymerase chain reaction (abbreviated PCR), allowing for more in-depth analysis.

What are the three main PCR steps?

For any DNA synthesis reaction, there are only three easy stages that must be completed: First, the template is denaturated into single strands. Next, primers are annealed to each original strand to synthesise new DNA strands. Finally, the new DNA strands are extended from the primers.

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