Question

This gene encodes a protein and you would like to determine whether the protein localizes to the nucleus or the cytoplasm. how would you examine where the protein localizes in the cells?

Answer 1

Protein subcellular expression and molecular complexing interactions are correlated with their functional activities. With the use of fractionation techniques or fluorescence microscopy-based techniques, localization may be efficiently established.

When a signaling route is understood, it is possible to deduce a protein's function from its location. To comprehend basic biological processes, fluorescent proteins can be overexpressed inside of cells and recombined with either target proteins or regulatory areas.

Cellular protein localization is widely studied using fluorescence microscopy. Normally, time-consuming and error-prone conventional methods are used to assess images of protein sites. Therefore, automated methods are necessary for the categorization of such photos.

These techniques must be precise and efficient in terms of computing.

To know more about protein signalling go to brainly.com/question/22082820

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