<u>Answer</u>:
1. Type of medium - the nutrient availability is one of the vital factors for the growth of a bacterial colony. Thus, the used growth medium will fulfill this requirement.
2. Incubation temperature - all bacteria have an optimal temperature range in which maximum growth is achieved. Any variations from this will result in negative effects on the amount of bacteria that are produced.
3. Moisture - bacteria thrive in moist or wet environments. Thus, the availability of moisture is another vital factor for the growth of the colony.
<span>The first law of thermodynamics is follows the quantity dQ = dW + dU where Q is the heat flow, W is the work and U is the internal energy. Only the internal energy is a state function where it means that it does not follow a specific path to arrive at a certain number. Whereas the work and heat are not state functions and they follow a specific path or process in order to arrive at a certain number. An example would be if y</span>ou are given 2,380 J of energy to do work as 12,900 J and you are to find the internal energy. Apply the equation above:
Q = U + W
2,380 J = U + 12,900 J
U = 15,280J.
