Answer:
In the decolorization step alcohol is used which destain the gram-negative bacteria but not gram-positive because alcohol degrades the LPS cell wall of gram-negative bacteria which allow the crystal violet to moves out of the cell.
So if we use too little alcohol then the alcohol will not able to decolorize all the gram-negative bacteria and most of the cell in the culture will appear violet or purple because crystal violet retained in the cell wall of bacteria.
So the sample of gram-negative bacteria will appear as gram-positive bacteria under the microscope.
Scientists believe the earth is approximately 4 1/2 billion years old
Take a compound microscope for example, the advantage of this common tool is to magnify a smaller object to make it view clearly. However the disadvantage is if you roll up the course adjustment knob too much the lenses will crack and you will have to give up some money.
