1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Alekssandra [29.7K]
3 years ago
10

Sequence the steps, 1–4, in the order required to create a permanent slide of human cheek cells. [1 mark] Specimen dehydrated Sp

ecimen embedded in wax Specimen cut into thin 'sections' Staining
Biology
1 answer:
gogolik [260]3 years ago
7 0

Answer:

The correct order is dehydration, embed in wax, cut into sections, staining

Explanation:

There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.

These steps are:

  • Obtention of the material: The tissue is cut to an adequate size.
  • Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.  
  • Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
  • Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.  
  • Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about  5 to 20 micrometers of thickness.
  • Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.  
You might be interested in
Photosynthesis also relies on a compound called __________,(ATP/NADP+/GLUCOSE/SOURCE/CARRIER/DONOR) which is an electron _______
grin007 [14]

Answer:

Photosynthesis also relies on a compound called NADP

which is an electron carrier accepts and transfers high-energy electrons. *

4 0
2 years ago
Genetic engineering has produced goat milk containing proteins that can be used as medicines. this effect was produced by
Kruka [31]
Inserting foreign genes into fertilized goats eggs
6 0
3 years ago
Which of the following is true about the products formed during photosynthesis?
Elina [12.6K]
Should there be answer choices that go with this question?

If not here is an answer that may help.

This is how the cycling between photosynthesis<span> and cellular respiration occurs: </span>in photosynthesis<span>, carbon dioxide and water, </span>in<span> the presence of light energy, are converted to make glucose and oxygen.</span>
8 0
3 years ago
Read 2 more answers
If molecules are small enough then
kakasveta [241]

sorry what this means¯\_(ツ)_/¯

6 0
3 years ago
Can someone help me with this pls
sladkih [1.3K]

Answer:

your answer is antibodies reacting to foreign antigens.

3 0
3 years ago
Read 2 more answers
Other questions:
  • .   Which variable stars have pulsation periods between 1.5 hours and 1.2 days? 
    14·2 answers
  • 2. What is the theory of inflation? How does it provide evidence for the<br> multiverse?
    7·1 answer
  • In general, what are three ways in which minerals form
    5·1 answer
  • What did raymond cattell call the underlying traits that direct surface traits?
    14·2 answers
  • Which statement below best summarizes the
    9·1 answer
  • Which statement is true for all prokaryotic and eukaryotic organisms?
    10·1 answer
  • Which two important factors affect cell differentiation? A. Environmental influences and cytoplasmic determinants B. Sperm bindi
    15·1 answer
  • Which of the following organelles uses carbon dioxide to produce sugars (glucose)?
    13·1 answer
  • Answer Correctly For 100 Points And Brainliest! Wrong Answers Will Be Reported
    15·2 answers
  • If water is at 26 degrees c and has a salinity of ____, its density is 1.023 g/cm3
    9·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!