Answer:
The correct answer is b.Amplify the gene using PCR. Insert the gene into a plasmid vector. Transform the vector into the bacteria.
Explanation:
If I have a very small amount of gene for a fluorescent protein than the first step is to amplify the gene so that appropriate protein can be produced. PCR is the instrument that is used to amplify the protein.
So after amplification of the gene, the plasmid vector will be used in which the gene will be inserted because the plasmid vector is used to insert this gene in host cells where protein will be expressed.
The final step will be to transform bacteria with recombinant plasmid so that plasmid can make its copy and express a fluorescent protein in bulk.
C. "connect the brain to spinal cord"
hope this helps
The correct answer is: A checkpoint will be activated if the spindle does not attach to a kinetochore.
Prokaryotes, do not undergo mitosis (like eukaryotes) and therefore have no need for a mitotic spindle. Prokaryotes also don’ t have checkpoints foor the regulation of cell division.
Normal eukaryotic cells (unlike cancer cells), move through the cell cycle in a regulated way in order to make sure that cells don't divide under conditions that are unfavorable for them. Information about their own internal state (nutrients, signal molecules, DNA integrity) is signal to go or not to go through the cell division. Because of that there are few checkpoints in the cell cycle at which the cell examines the signals and makes a “decision”. The major checkpoints are:
• The G1- the first point at which it must choose, once it passes the G1 checkpoint the cell enters S phase
• The G2-the cell checks DNA integrity and checks if replication is done well.
• The spindle checkpoint-at the transition from metaphase to anaphase.
The temperature is lowest at the crust. As you get lower into the Earth's surface, the temperature gets hotter.
