Answer:Chromatography is actually a way of separating out a mixture of chemicals, which are in liquid or gas form, by letting them flow slowly past another substance, which is either a solid or a liquid. It consist of a stationary phase and a mobile phase.
Explanation: All chromatographic systems rely on the fact that a substance placed in contact with two unmixable phases, one movable phase and one stationary phase, will equilibrate between them. A selectivity (or separation) factor (α) is used to 'chemically' distinguish between sample components. It is usually measured as a ratio of the retention (capacity) factors (k) of the two peaks in question and can be visualized as the distance between the apices of the two peaks. reproducible fraction will partition into each phase, depending on the relative affinity of the substance for each phase. A substance which has affinity for the moving or mobile phase will be moved rapidly through the system. A material which has a stronger affinity for the stationary phase, on the other hand, will spend more time immobilized in that phase, and will take a longer time to pass through the system. Therefore, it will be separated from the first substance. By definition, chromatography is a separation technique in which a sample is equilibrated between a mobile and a stationary phase. A theoritical plate or tray is used to produces the best possible difference between the liquid and vapour phases in equilibrium with it
Chromatographic separations are best done with a small amount of analyte (substance to be separated during analysis), which keeps either phase from becoming saturated with analyte, so that the concentrations in the two phases are directly proportional. Overloading the column with sample causes one of the phases to become saturated, leading to a loss of column efficiency, and poorly shaped peak profiles.
The retention volume in chromatographic separation (Vr) is the volume of the mobile phase required to carry the solute through the column to elution, is related to the column flow (Fc) and the retention time (tr). Likewise, the volume of the mobile phase(Vm), is related to the flow and the time the void volume takes to pass through the column.
Band broadening using the kinetic model is a phenomenon that reduces the efficiency of the separation being carried out, leading to poor resolution and chromatographic performance. This is problematical in terms of both the quality of the separation obtained and the accuracy with which sample components can be quantified.The wider band results in a dilution effect that produces a decrease in peak height accompanied by a loss in sensitivity and resolution. The eddy dispersion, accounts for the source of band broadening related to any flow unevenness in the column.
The branch of biology that deals with the relations of organisms to one another and to their physical surroundings.
The term scientific methodology has to do with the process by which knowledge is acquired in science.
<h3>What is scientific methodology?</h3>
The term scientific methodology has to do with the process by which knowledge is acquired in science. This process usually involves the heavy use of experimentation.
First the scientist carries out an observation which leads to the propounding of a hypothesis which is then tested by experiment before it could be accepted as a fact.
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Answer:
Enzymes are one kind of protein which functioning as catalyst that speed up reactions by lowering activation energy.Enzyme accelerate a reaction without altering its chemical equilibrium.
Explanation:
Energy which is required for start a biochemical reaction is called activation energy.Activation Energy helps to jump and start a thermodynamically favorable reactions.
Enzymes can many way to its activation energy.
1. The enzyme may hold the substrates in such a way as to distort the substrate bonds closer to their form in the transition state. This reduces the amount of energy needed to complete the transition.
2.Enzyme create a charge distributor which opposite of transition state his lowers the energy of the transition state and decreases the activation energy.
3.The enzyme may reduce the reaction entropy by bringing substrates together in the correct orientation to react.
4. The enzyme may provide a completely different chemical pathway for the reaction. It may form new bonds in the ES complex that would be difficult to form without the enzyme.
The answer to this question would be: visceral motor
The muscle in the stomach is not consciously moved. The muscle regulated automatically by the nervous system based on some mechanism like other organs. The things that can influence the muscle would be when the autonomous nervous system is activated. This kind of motor innervation is called visceral motor.
