It would be the last one.
For example, pH can have an effect of the state of ionization of acidic or basic amino acids. Acidic amino acids have carboxyl functional groups in their side chains. Basic amino acids have amine functional groups in their side chains. If the state of ionization of amino acids in a protein is altered then the ionic bonds that help to determine the 3-D shape of the protein can be altered. This can lead to altered protein recognition or an enzyme might become inactive.
Changes in pH may not only affect the shape of an enzyme but it may also change the shape or charge properties of the substrate so that either the substrate connot bind to the active site or it cannot undergo catalysis.
In geneal enzyme have a pH optimum. However the optimum is not the same for each enzyme.
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Some species are more likely to perish but, the most healthy frogs will most likely evolve and find something else to eat.
Answer:
f = m - u
Explanation:
DNA methylation is an important epigenetic modification associated with the regulation of gene expression. As such, the analysis of DNA methylation marks and the dynamics of this process may shed light on the genes that are being expressed at a given time in a given cell type/tissue. In the example above described, f is defined as the fraction of the DNA molecule that is methylated after one (1) hour, whose value can be estimated by subtracting u fraction (i.e., fraction of methylated locations that become unmethylated) from m fraction (i.e., fraction of unmethylated locations that become methylated).
In consequence, the equation is
f = m - u
