Cellular respiration uses energy in glucose to make ATP. Aerobic (“oxygen-using”) respiration occurs in three stages: glycolysis, the Krebs cycle, and electron transport. In glycolysis, glucose is split into two molecules of pyruvate.The net energy gain from one cycle is 3 NADH, 1 FADH2, Page 4 Cellular respiration 4 and 1 GTP; the GTP may subsequently be used to produce ATP. Thus, the total energy yield from one whole glucose molecule (2 pyruvate molecules) is 6 NADH, 2 FADH2, and 2 ATP.
Cellular respiration
Extraction, homogenization, and centrifugation are the three processes in cell fractionation.
Cell fractionation is a method for dividing cellular components while yet maintaining each component's unique functionality. This technique was initially applied to show where distinct biochemical activities are located within cells. Subcellular fractionation is also used to help with illness detection and to offer an enhanced source of protein for further purification. To prevent osmotic damage, tissue is routinely homogenized in an isotonic buffer solution. Grinding, mincing, chopping, pressure changes, osmotic shock, freeze-thawing, and ultrasonic waves are a few of the mechanisms for homogenization. After that, the samples are maintained cool to avoid enzymatic deterioration. It is the development of a uniform mass of cells (cell homogenate or cell suspension).
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Answer:
ROY G. BIV is an acronym used to memorize the colors on the EM spectrum. It stands for red, orange, yellow, green, blue, indigo, and violet.
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