Gram's staining is a differential staining technique that employs a primary stain like crystal violet and a counter stain like safranin along with the decolourizing agent alcohol and a mordant called the Gram's iodine.
Iodine is a mordant added after the primary stain. It fixes the stain by combining with it to enchance the staining ability. This forms an insoluble crystal violet iodine complex appearing purple under the microscope. These microorganisms are classified as Gram positive.
If addition of iodine is skipped, crystal violet is not fixed on the slide and the insoluble complex is not formed. The cells are decolourized by alcohol and are stained by the counter stain safranin making the Gram positive cells wrongly indentified as Gram negative due to its pink colouration. Thus, the slide will show all the cells as pink coloured Gram negative cells.
Answer and Explanation:
A null hypothesisstates that there is no significant difference between specified populations, any observed difference being due to sampling or experimental error.
In order to reject or retain a null hypothesis, compare the P-value to the alpha . If the P-value is less than (or equal to) ,the null hypothesis is rejected in favor of the alternative hypothesis. If the P-value is greater than , the null hypothesis is not rejected.
Answer:
<h3>MAP OF THE PHILIPPINES</h3>
Explanation:
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DNA is an acid that contains resources needed for development and function of every living thing, EVERY LIVING THING. even bacteria and viruses have DNA. without DNA there would be no life.
