It helped be able to look more closely at the the cells
The absorbance reported by the defective instrument was 0.3933.
Absorbance A = - log₁₀ T
Tm = transmittance measured by spectrophotometer
Tm = 0.44
Absorbance reported in this equipment = -log₁₀ (0.44) = 0.35654
True absorbance can be calculated by true transmittance, Tm = T+S(α-T)
S = fraction of stray light = 6%= 6/100 = 0.06
α= 1, ideal case
T = true transmittance of the sample
Tm = T+S(α-T)
now, T= Tm-S/ 1-S = 0.44-0.06/ 1-0.06 = 0.404233
therefore, actual reading measured is A = -log₁₀ T = -log₁₀ (0.404233)
i.e; 0.3933
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Explanation:
Molar mass
The mass present in one mole of a specific species .
The molar mass of a compound , can easily be calculated as the sum of the all the individual atom multiplied by the number of total atoms .
(a) S₈
Molar mass of of the atoms are -
sulfur, S = 32 g/mol.
Molar mass of S₈ = 8 * 32 g/mol. = 256 g/mol.
(b) C₂H₁₂
Molar mass of of the atoms are -
Hydrogen , H = 1 g/mol
Carbon , C = 12 g/mol
Molar mass of C₂H₁₂ = ( 2 * 12 ) + (12 * 1 ) = 36 g /mol
(c) Sc₂(SO₄)₃
Molar mass of of the atoms are -
sulfur, S = 32 g/mol.
oxygen , O = 16 g/mol.
scandium , Sc = 45 g/mol.
Molar mass of Sc₂(SO₄)₃ = (2 * 45 ) + ( 3 *32 ) + ( 12 * 16 ) = 378 g /mol
(d) CH₃COCH₃ (acetone)
Molar mass of of the atoms are -
Carbon , C = 12 g/mol
oxygen , O = 16 g/mol.
Hydrogen , H = 1 g/mol
Molar mass of CH₃COCH₃ (acetone) = (3 * 12 ) + ( 1 * 16 ) + ( 6 * 1 ) = 58g/mol
(e) C₆H₁₂O₆ (glucose)
Molar mass of of the atoms are -
Carbon , C = 12 g/mol
oxygen , O = 16 g/mol.
Hydrogen , H = 1 g/mol
Molar mass of C₆H₁₂O₆ (glucose) = ( 6 * 12 ) + ( 12 * 1 ) + ( 6 * 16 ) = 108g/mol.
