When organisms use urea, ammonia is generated during incubation, which makes the reaction of these media alkaline
Explanation:
Urea broth preparation:
Suspend 3.87 grams of the medium in 100 ml of distilled water without heating. When the powder dissolves, sterilize by filtration. Dispense quantities of 0.5 to 2 ml in small sterile tubes. Larger volumes may be used but the reactions will be slower. Do not sterilize in autoclave.
Do not boil the medium.
If no filter is available, the medium can be sterilized at 100-110 ° C for 10 minutes. If the medium is prepared and inoculated immediately, it provides good results without sterilization.
If there is a temperature difference between two objects, the object that has a higher temperature will want to level out its heat. Therefore, it will transfer the heat it needs to the other object if it allows until they are the same temperature.
