Answer:
Each mutant would be mated to wild type and to every other mutant to create diploid strains. The diploids would be assayed for growth at permissive and restrictive temperature. Diploids formed by mating a mutant to a wild type that can grow at restrictive temperatures identify the mutation as recessive. Only recessive mutations can be studied using complementation analysis. Diploids formed by mating two recessive mutants identify mutations in the same gene if the diploid cannot grow at restrictive temperature (non-complementation), and they identify mutations in different genes if the diploids can grow at restrictive temperature (complementation).
Explanation:
Recessive mutations are those whose phenotypic effects are only visible in homo-zygous individuals. Moreover, a complementation test is a genetic technique used to determine if two different mutations associated with a phenotype colocalize in the same <em>locus</em> (i.e., they are alleles of the same gene) or affect two different <em>loci</em>. In diploid (2n) organisms, this test is performed by crossing two homo-zygous recessive mutants and then observing whether offspring have the wild-type phenotype. When two different recessive mutations localize in different <em>loci</em>, they can be considered as 'complementary' since the heterozygote condition may rescue the function lost in homo-zygous recessive mutants. In consequence, when two recessive mutations are combined in the same genetic background (i.e., in the same individual) and they produce the same phenotype, it is possible to determine that both mutations are alleles of the same gene/<em>locus</em>.
1. Water is abioitic and is needed by every living organism Soil is abioitic and is needed by plants Trees and other plants release water vapor from their leaves (a process called transpiration) that create humidity (which in turn influences how much rain falls in an area) The climate in an area influences the special adaptations that plants and animals have. For example: warm fur coats and thick layers of fat to keep warm in cold climates, animals in dry, hot climates (desert) have large ears to release heat and cool down. Biotic factors also influence abiotic factors. Animals produce waste (go to the bathroom) which in turn will become nutrients in the soil.
2. decrease heat will affect biotic factors, like animals, warmth.
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Answer:
Scientific theories can never be proven true beyond all doubt; they can only be supported by a wide body of evidence. Only one of the statements that follow uses the term theory in its correct, scientific sense.
Explanation:
Answer:
Primers should be:
3 'ACGTATGTCGCTTAGT 5'
5 'ACGTTCCAGTCAATGA 3'
Explanation:
DNA replication occurs with the need for the presence of primers that are a small sequence of nitrogenous bases that will allow the initiation of replication.
Primers are extremely important in PCR and are represented as small pieces of single-stranded DNA that specify the region of DNA that should be replicated during PCR. These primers attach to the single strand of DNA by pairing the nitrogenous bases allowing the DNA polymerase to recognize where replication begins.
Regarding the question above, the primers should be:
3 'ACGTATGTCGCTTAGT 5'
5 'ACGTTCCAGTCAATGA 3'
