Roche/454 pyrosequencing , useful for high throughput sequencing techniques. This method utilizes pyrosequncing which allows for sequencing as the sequence read out can be achieved at the same time as the sequence is extended.
High- throughput screening is the use of automated equipment to rapidly test thousand to millions of samples for the biological activities at the model organism, cellular, pathway or molecular level.Many DNA samples can be sequenced simultaneously.
Sanger DNA sequencing also known as chain termination. This method is used only for the single DNA fragment at a time. Amplicon sequencing with NGS is efficient and cost effective . It is required a DNA polymerase , nucleotide , dideoxynucleotides and buffer.
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Answer:
Only P-, F-, and V-class pumps transport ions.
Explanation:
The distinct classes of ATPases include:
1) Only the P-type ATPase actively transports ions across biological membranes. P-ATPases (also named E1-E2 ATPases) are found both in plasma and organelle membranes. These ATPases serve to transport ions and phospholipids by hydrolyzing ATP to ADP and phosphate.
2) A- and F-ATPases synthesize ATP by transforming the energy from a gradient of ions across the cell membrane.
3) V-ATPase (also known as Vacuolar-H+ ATPases) acidifies vacuole, lysosome, endosome and Golgi membranes. This type of ATPase couples the hydrolysis of ATP to the active transport of protons across biological membranes.
4) E-ATPases hydrolyze extracellular ATP.
The genetic characteristic of two different organisms are induced into a new host organism for the purpose of producing new genes.
<u>Explanation:</u>
In the field of bio technology, Recombinant DNA technology plays a very important role. This helps in the production of genes. The first thing insulin of human was produced with this technology. In this technology, the gene that is to be produced is cut and it is placed inside a host organism where it gets multiplied.
This technology uses five steps in gene production. Firstly, the DNA that is essential will be cut. this is done by restriction site. PCR is used secondly in order to amplify the copies of the genes. Then these are induced into Vectors after which they will again be introduced into a host organism. Then finally the results will be the genes of the desired characteristics.
