If we imagine that plant has two alleles for the
trait, we can dominant allele represent with P represents and recessive allele with p. To get purebred monohybrid in the first generation, parents must be a dominant homozygote (PP) and a recessive hetero<span>zygote (pp):
Parental generation: PP x pp The first generation: Pp Pp Pp Pp
Pp represents a heterozygote. If we cross these heterozygotes:
The first generation: Pp x Pp The second generation: PP Pp Pp pp
If dominant allele determines the phenotype, there will be 3 plants (one PP and two Pp) with one phenotype and only 1 plant </span><span>(pp)</span> with another phenotype and vice versa.
Plasmid or vector transformation is the process by which exogenous DNA is transferred into the host cell. Transformation usually implies uptake of DNA into bacterial, yeast or plant cells, while transfection is a term usually reserved for mammalian cells.