He most likely suffers from sleep apnea.
Sleep apnea refers to a breathing disorder; sometimes people experience difficulties breathing or they completely stop breathing during sleep which lasts for a couple of seconds but still creates discomfort for the person suffering from it. It can occur a couple of times during sleep and is followed by snoring, which is a common thing for Pierre in this example.
According to Weston, people who equated their adoption of a lesbian or gay identity with a renunciation of family did so in the double-sided sense of fearing rejection by the families in which they had grown up, and not expecting to marry or have children as adults.
According to Kath Weston, an anthropologist, in the book The Families We Choose: Lesbians, Gays, Kinship discusses the adoption of a lesbian or gay identity with a renunciation of family. Even though gays and lesbians enjoy an unprecedented degree of acceptance, many gay and lesbian still adopt the popular equation of their sexual identities with the renunciation of access to kinship. They equated their adoption of a lesbian or gay identity with a renunciation of family did so in the double-sided sense of fearing rejection by the families in which they had grown up, and not expecting to marry or have children as adults.
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The Justification Defense:
Justification defenses include Necessity<span>,Defense of others, Defense of property, Law Enforcement Defense, Consent. Excuse defenses include </span>Duress<span>, </span>Entrapment<span>, Ignorance of the Law, Diminished </span>Capacity Defense<span>, </span>Provocation<span>, </span>Insanity Defense<span>, and </span>Infancy Defense<span>.</span>
1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
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