Answer:
D) Three of the reaction steps in gluconeogenesis would have prohibitively large, positive free energies if they used glycolytic enzymes for their catalysis.
Explanation:
The glycolytic enzymes catalyze the conversion of glucose to pyruvate, while gluconeogenesis enzymes catalyze the formation of carbohydrates from pyruvate.
An eon is the most inclusive
I think it's the last two
Temperature and the amount of water