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Elina [12.6K]
3 years ago
5

When an organism has no chlorophyll, uses binary fission and has a non-organized nuclear body the organism is a?

Biology
1 answer:
Yuliya22 [10]3 years ago
6 0
It is a prokaryotic cell. 
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One of your lab partners has followed the recommended procedure of running Gram-positive and Gram-negative control organisms on
andreyandreev [35.5K]

Answer:

Reduced in holding time of decolrization step and also used less Alcohol because decolrization step is important in Gram's staining.The decolorization step must be performed carefully. Otherwise over-decolorization may occur. This step is critical and must be timed correctly otherwise the CV stain will be removed from the Gram-positive cells. If the decolorizing agent is applied on the cell for too long time, the Gram-positive organisms to appear Gram-negative..

Explanation:

Gram' staining is a technique used in microbiology labs to differentiate between Gram's positive and negative

<u>Gram-positive bacteria :</u>Stain dark purple due to retaining the primary dye called CV in the cell wall.

<u>:Gram-negative bacteria </u>Stain red or pink due to retaining the counter staining dye called Safranin or neutral red.

There are four basic step in Gram" staining

1) Application of the Primary Stain to a Heat-Fixed Smear of Bacterial Culture

2)Addition of Gram's Iodine

3)<u>Decolorization with 95% Ethyl Alcohol:</u>Alcohol or acetone dissolves the lipid outer membrane of Gram-negative bacteria, thus leaving the peptidoglycan layer exposed and increases the porosity of the cell wall. The CV-I complex is then washed away from the thin peptidoglycan layer, leaving Gram-negative bacteria colorless.

On the other hand, alcohol has a dehydrating effect on the cell walls of Gram-positive bacteria that causes the pores of the cell wall to shrink. The CV-I complex gets tightly bound into the multi-layered, highly cross-linked Gram-positive cell wall thus staining the cells purple.

The decolorization step must be performed carefully. Otherwise over-decolorization may occur. This step is critical and must be timed correctly otherwise the CV stain will be removed from the Gram-positive cells. If the decolorizing agent is applied on the cell for too long time, the Gram-positive organisms to appear Gram-negative. Under-decolorization occurs when the alcohol is not left on long enough to wash out the CV-I complex from the Gram-negative cells, resulting in Gram-negative bacteria to appear Gram-positive.

7 0
3 years ago
A current federal program from the New Deal that is directly related to the banking industry is the
masha68 [24]
The answer is FDIC (Federal Deposit Insurance Company)

The current federal program from the New deal that is directly related to the banking industry is the FDIC. This program examines and supervises financial institutions on some factors such as safety and soundness. It promotes and preserves public confidence in the State's financial system.
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3 years ago
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Why is the frequency of recombinant gametes always half the frequency of crossing over?
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Crossing over occurs at the four-strand stage when two homologous chromosomes, each consisting of a pair of sister chromatids, are paired. Each crossover involves just two of the four strands and generates two recombinant strands.

Hope this helps!
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What is puberty for boys?
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When boys go through puberty their voices change and they start to grow hair on different area of their body such as their chest, face and pubic area. Also they will start to get acne

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