Answer:
D
Explanation:
The simple answer is the electrons in the outermost energy level.
Hydrogen has 1 electron in the outermost energy level.
Magnesium has 2 so this tells you that magnesium has a charge of 2
Oxygen has 6 oxygen has a charge of - 2
Fluorine has 7
For most elements, the electrons in the most outer ring determine the valence of the element.
Notice that the non metals work differently than the metals. Mg may have a charge of 2 and that is the number of electrons in the valence right.
Oxygen is a non metal it has a charge of - 2. It gets 6 electrons by subtracting the number of its charge from 8.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Statement B is false.
Explanation:
PKA and PKG is both have binding domains to attach with nucleoside monophosphate and help in modulating these enzymes that explains that PKA is more related to the PKG than PKC at the level of amino acid.
At the level of polypeptide chain level PKG has single polypeptide chain whereas in PKA modulatory domains are located on different poly peptide chain. Both are activated by the nucluoside triphosphate, more precisely PKA by cAMP and PKG is by cGMP respectively, whereas PKC is activated by DAG or Ca or both depending on the isofom.
Thus, the correct answer is - option B.
i would say the best answer is d
