Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
1. C) Change in fossil fuel use
2. C) Reduce the amount of fossil fuels used
The main problem that your central nervous system will try to solve is how to get out of this place.
<h3>B. What is the somatic nervous system probably doing?</h3>
It is releasing stimuli so that the pupil dilates and the blood goes to the muscles, to give greater physical vigor.
<h3>ç. Which part of the autonomic nervous system is likely to be active and what is it doing?</h3>
The autonomic nervous system is a part of the nervous system that functions independently of will and consists of neurons that conduct impulses from the central nervous system (brain and/or spinal cord) to the glands, smooth muscle and heart muscle.
<h3>What are the physiological responses of adrenaline binding in the sympathetic autonomic system?</h3>
Adrenaline increases the overall activity of the heart, increasing both the heart rate and the force of contraction. The heart has β1 receptors in both contractile and specialized myocardium. When turning on, a series of cardiac effects can happen.
With this information, we can conclude that the main problem that your central nervous system will try to solve is how to get out of this place.
Learn more about central nervous in brainly.com/question/17520523
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Could you add a little more to this question, please?
Ok so alison spends the morning then her desk is the desk
