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lesya [120]
3 years ago
8

You analyze a dna sample which gives an a260 of 1.74 and a280 of 0.64. What is the concentration of this dna sample? Is it suffi

ciently pure?
Biology
1 answer:
LenaWriter [7]3 years ago
8 0

The ratio of \frac{A_{260}}{A_{280}}  indicates the purity of nucleic acid.For pure DNA it should lie between 1.8-2.0.Whereas for RNA it should be greater than 2.

This is calculated for DNA using the given values of absorbance-

\frac{A_{260}}{A_{280}}= 2.71

As the value is greater than 2.0,it reflects the RNA contamination in the sample.

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Answer:

The birth rate refers to an average number of births in one year per thousand individuals in the population at mid-year.  

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BR = (50000 / 1000000) * 1000 = 50

Death rate refers to an average number of death in an annum per thousand individuals in a population at mid year. Thus,  

DR = (total death / total population in mid year) * 1000

DR = (10000 / 1000000) * 1000 = 10

Annual population growth rate or APGR is calculated as:  

APGR = (Birth rate - Death rate) / Death rate

APGR = (50 - 10) / 10 = 4.0 %

Doubling time is the whole sum time needed for a population to get double in size. It is calculated as DT = ln (2) / growth rate

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Thus, population will get double in 17.5 years.  

Adjusted (true) growth rate is equal to APGR + (NMR / Death rate)

And NMR = [(immigrants -emigrants) / total population] * 1000

NMR = [(5000 - 20000) / 1000000] * 1000 = -15

Thus, Adjusted (True) growth rate = 4.0 + (-15 /10) = -6%

The adjusted (true) doubling time = ln (2) / Adjusted growth rate  

= 70 / -6 = -11.66

This negative sign shows that it will never get double in such situation.  

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