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lesya [120]
3 years ago
8

You analyze a dna sample which gives an a260 of 1.74 and a280 of 0.64. What is the concentration of this dna sample? Is it suffi

ciently pure?
Biology
1 answer:
LenaWriter [7]3 years ago
8 0

The ratio of \frac{A_{260}}{A_{280}}  indicates the purity of nucleic acid.For pure DNA it should lie between 1.8-2.0.Whereas for RNA it should be greater than 2.

This is calculated for DNA using the given values of absorbance-

\frac{A_{260}}{A_{280}}= 2.71

As the value is greater than 2.0,it reflects the RNA contamination in the sample.

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