Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Answer:
Viruses needs to inject the virus to an organism's body, or host cell and duplicate as many viruses, so it can be able to insert it's own clones and take over the body sooner :3
Explanation:
:3
The Add to Dictionary function in Meg's word processor will remove the highlight of that word as a spelling mistake.
In ecology, the law of conservation of mass is applied by the principle of taking energy from the lower trophic levels and passing it to the higher trophic level by any means.
<h3>What is the law of conservation of mass?</h3>
The law of conservation of mass states that the actual mass in an ecosystem is neither created nor destroyed. Overall remains constant in a system.
In ecology, it illustrates that when an organism is ingested by other organisms, its mass is conserved. Some of the organisms may also be dependent on the waste products like urine and feces to make proteins and other compounds.
Therefore, the energy or mass is neither lost in any form. It simply is taken by other organisms through any means.
To learn more about Ecological principles, refer to the link:
brainly.com/question/7413811
#SPJ1
