Answer:
B
Explanation:
The lung not being not been able to exchange and remove the carbon dioxide created a health problem due to the concentration of hydrogen ions (H+) being 10X being higher than in normal body. Although the difference is just about 1, it will result increase hydrogen ion concentration 10 times, similarly an increase or decrease in pH from 4 to 2 will give rise to 100 times increase in hydrogen ion concentration. It can equally be applied to hydroxyl in concentration.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
This qualifying core is an area with a population density of at least 1,000 people per square mile that contains at least 50 percent of Page 8 12-8 Urban and Rural Classifications the place's total population and is contiguous with other qualifying urbanized territory that also meets the population density criterion.
