Nicked circle is the reason.
Explanation:
During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.
Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.
The ligation in the plasmid did not occur properly hence the replication is interrupted hence no new plasmids with gene of interest replicated in the ligation carried out.
The answer is true. A matches with T. C matches with G.
If you remove a secondary consumer then, primary consumers have the chance to overpopulated and Tertiary consumers underpopulate or die off because there's no secondary consumers to eat
