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Stells [14]
3 years ago
8

Four restriction digestion reactions were performed using the plasmid containing the ligated pcr product and one or more restric

tion enzymes. the result of each restriction digest was then run on an agarose gel. use the plasmid map from part e to help you determine which restriction enzymes were used for each reaction.
Biology
1 answer:
valentina_108 [34]3 years ago
6 0

Where is the plasmid map
Maybe because of the gel

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Problem #1
Arisa [49]

Answer:

9/16  Kinked tail, normal growth

3/16 Kinked tail, obese growth

3/16 normal tail, normal growth

1/16 normal tail, obese growth

Explanation:

Kinked tail (K) is dominant over normal tail (k) and normal growth (N) is dominant over obese growth (n). Two mice that are heterozygous for both traits (KkNn) are crossed:

        KkNn    x    KkNn

offspring:

<em>9/16</em><em> K_N_ - Kinked tail, normal growth</em>

<em>3/16</em><em> K_nn - Kinked tail, obese growth</em>

<em>3/16</em><em> kkN_ - normal tail, normal growth</em>

<em>1/16</em><em> kknn - normal tail, obese growth</em>

(See the attached image for the Punnet's square analysis)

3 0
3 years ago
Which is an example of genetic engineering??
RSB [31]

Answer:

cloning

Explanation:

one of the most controversial uses of genetic engineering has been cloning, or producing a genetically identical copy of organism. while the ethics of cloning are hotly debated, the 1st ever sheep was cloned in 1996 by scientists.

4 0
4 years ago
A plasmid _____________________________ . A. can confer antibiotic resistance to a bacterium. B. is a single-stranded circular D
hodyreva [135]

Answer:

The correct answer will be option A.

Explanation:

The bacteria organize its genetic material in the single circular chromosome suspended in the cytosol along with additional circular double-stranded DNA molecule called plasmid.

The plasmid is present as a separate structure in the cytoplasm which performs important functions during stressful conditions in the bacteria like providing antibiotic resistance to the bacteria, formation of filial factors during conjugation and many others.

The plasmid which confers resistant to the bacteria is known as the R-plasmid. The R-plasmid consists of r-determinants which confers antibiotic resistance to the bacteria and resistance transfer factor (RTF) which helps in plasmid transfer.

Thus, option A is the correct answer.

7 0
3 years ago
Agriculture led to an increase in the size of the human population. Which of the following was the most important impact of agri
klemol [59]

Answer:

The answer is the first option.

Explanation:

Farming emerged because being a nomad was no longer suitable for increasing population size. Farming allowed people to stay in one place and properly feed their families, which was a definite positive impact on population size.

8 0
3 years ago
Whose main job is to give strength and stiffness to a plant cell?
Artyom0805 [142]

Answer:

cell wall

Explanation:

4 0
3 years ago
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