1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Thepotemich [5.8K]
2 years ago
8

Describe how a plasmid can be genetically modified to include a piece of foreign dna that alters the phenotype of bacterial cell

s transformed with the modified plasmid. describe a procedure to determine which bacterial cells have been successfully transformed.
Biology
1 answer:
Ilia_Sergeevich [38]2 years ago
7 0
Simple version:

First, the section with the desirable gene must be identified. Assuming that has already happened, the section of DNA must be excised from the original genome using restriction enzymes, which recognize certain DNA sequences and snip DNA at those sites. DNA ligase is used to "glue" these ends back together. The DNA is inserted into a plasmid (also with restriction enzymes), which would usually contain antibiotic-resistance genes (so they survive in an environment containing the antibiotic, which would also help show if the bacteria have been successfully transformed). 

Then comes the actual transformation process. The bacteria to be transformed are mixed with calcium chloride (which causes the bacteria to be more receptive to the plasmids) and then mixed with the plasmids. The bacterial cells are subjected to a heat shock (the solution is heated and rapidly cooled, e.g. by placing the mixture in a hot water bath and quickly transferred to ice) so they will take up the plasmid (since the temperature change makes the membrane more permeable). The bacteria are placed on a growth medium containing the antibiotic they're resistant to. Only those successfully transformed would survive.
You might be interested in
When you are on the roller coaster at Carowinds, your adrenal cells near the kidneys
kicyunya [14]

Answer:

D. Cell signalling which is released by one type of cell and acts upon a different cell

Explanation:

According to this question, adrenal cells near the kidney releases an hormone called ADRENALINE, which causes the cells of the heart to beat faster. This is an example of CELL SIGNALLING because chemical signals released by adrenal glands causes/triggers a response in the heart cells.

The chemical signal released by the adrenal cells is the ADRENALINE HORMONE, which induces a response in cells that are different from the cells of release (adrenal cells).

5 0
2 years ago
11. Which is a density-dependent factor?
Sophie [7]

Answer:

Explained below:

Explanation:

Density-dependent factors are biological factors adopted by the population as a resource. The things can be like shelter, food, or different poor resources. Density-dependent factors affect fitful variations in the population as its density fluctuations. If the population is small, these portions typically support improved rates of birth and lower will be the death rates, empowering the population to increase and when the population is big and thick, these factors display that the birth rate is decreased and death rate became higher.

6 0
3 years ago
Most stars end their lives as which type of star?
mestny [16]
A white dwarf is the answer
6 0
3 years ago
3)
lisabon 2012 [21]

Answer:

A) climate change...........

4 0
3 years ago
Why is 2 the correct answer to number 16 ? Explain why please and answer this !!
marta [7]

an egg cell has a haploid nucleus so it has half the number of dna because it fuses with a sperm cell to have the full amount of dna.

an egg cell has 23 chromosomes and a sperm cell has 23 chromosomes.

they both have half the amount of dna to form a zygote

5 0
2 years ago
Other questions:
  • What role does heat energy from the Sun play in influencing ocean currents?
    13·1 answer
  • In which digestive system organ will you find the enzymes responsible for breaking down disaccharides into monosaccharides and d
    13·1 answer
  • Which of these statements explains a key difference between unicellular and multicellular organisms?
    6·1 answer
  • Who first convinced this idea about gravity force?
    5·2 answers
  • Where does translation occur?
    15·1 answer
  • Prenatal detection of chromosomal abnormalities is accomplished chiefly by
    12·1 answer
  • What does it mean when a compunds chemical formula does not include any numbers?​
    10·1 answer
  • If a species makes up a small portion of the community by weight, yet exerts a disproportionate influence on community diversity
    6·1 answer
  • ana walked through this part of the forest. When she passed under some low branches, water drops fell on her head. What most lik
    8·1 answer
  • How can intensive agricultural practices contribute to climate change?
    8·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!