Question

Addition of dns at the end of the incubation period stopped the reaction by denaturing sucrase. explain why it is important to denature sucrase before measuring product concentration.was the rate of increase of sucrase activity greater when sucrose concentration went from 2.5 to 7.5 g/l or when it went from 22.5 to 27.5 g/l?

Answer 1

Because sucrase will react with product and lower his concentration, so measurement of product concentration will not be accurate.
Sucrase activity is greater when sucrose concentration went from 2.5 to 7.5 g/l, because difference (increase) between two concentration (numbers) is greater.