Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Ocean Thermal Energy Conversion (OTEC)
Explanation:
The correct statement is that the concentration of acetylcholine will be inhibited by enzymes and can cause several diseases as follows:
- Alzheimer's disease-related to damage to brain cells,
- memory loss,
- and thinking ability.
How to reduce the symptoms of these diseases is to use drugs that contain cholinesterase inhibitors.
<h2>Further Explanation
</h2>
Acetylcholine is a neurotransmitter that plays an important role in the functioning of the autonomic nervous system. Acetylcholine is one of the most commonly recognized neurotransmitters or nerve stimulating chemicals. These neurotransmitter compounds can be found in the nervous system.
There are several side effects of the acetylcholine drug, which are as follows:
- Can cause a lot of sweat
- Excessive salivation
- Stomach ache
- Headache
- Blood vessel dilation
- Nausea and vomiting
- Diarrhea is a sign of an increase in parasympathetic tone.
Learn More
- about chemicals brainly.com/question/8735339
- about enzymes brainly.com/question/6577765
Details
Grade: Middle School
Subject: Biology
Keyword: chemicals, enzymes, Acetylcholine, neurotransmitter
Earth's crust is made of rock, and rock is made of natural substances called minerals. There are three main types of rock: sedimentary, igneous, and metamorphic rock. Each type is produced in different ways. The oldest rocks on Earth were formed about 3.8 billion years ago.
So here let's first find the amount of children every santa, in total, sees in one day. So if there are 400 santas, and each sees 125 children, you can just multiply 400 by 125 to see how many children all the Santas see in one day.
400 * 125 = 50,000
Now, since there are 30 days which all the santas see 50,000 children, you can then multiply 30 by the 50,000 children to get how many children are seen by Macy's Santas in a month.
30 * 50,000 = 1,500,000
So 1,500,000 children are seen by Macy's Santas.
