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UkoKoshka [18]
3 years ago
10

You need to determine if a pure culture of bacteria is gram-positive or gram-negative, but you've just spilled your only solutio

n of crystal violet so you can't do a Gram stain. You decide to try a lysozyme treatment on a sample of each culture and then examine the samples under the microscope. The expected result is?
Biology
1 answer:
emmainna [20.7K]3 years ago
7 0

Answer:

Gram positive bacteria will be lysed whereas gram negative bacteria will be unaffected.

Explanation:

  • Lysozyme treatment degrades bacterial cell wall. It destroys the peptidoglycan cell wall by hydrolyzing the glycosidic bond between NAM and NAG sugar residues present in the cell wall.
  • Gram positive bacteria has a thick peptidoglycan cell wall with only a thin outer layer of techoic acid. So when exposed to lysozyme, the peptidoglycan layer disintegrates and forms debris.
  • Gram negative bacteria has a thin peptidoglycan cell wall and a tough outer membrane. The outer membrane does not allow the lysozyme to come in contact with the cell wall keeping it intact.
  • Hence, Gram positive bacteria will be lysed whereas gram negative bacteria will be unaffected by lysozyme treatment.
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