You need to determine if a pure culture of bacteria is gram-positive or gram-negative, but you've just spilled your only solutio
n of crystal violet so you can't do a Gram stain. You decide to try a lysozyme treatment on a sample of each culture and then examine the samples under the microscope. The expected result is?
Gram positive bacteria will be lysed whereas gram negative bacteria will be unaffected.
Explanation:
Lysozyme treatment degrades bacterial cell wall. It destroys the peptidoglycan cell wall by hydrolyzing the glycosidic bond between NAM and NAG sugar residues present in the cell wall.
Gram positive bacteria has a thick peptidoglycan cell wall with only a thin outer layer of techoic acid. So when exposed to lysozyme, the peptidoglycan layer disintegrates and forms debris.
Gram negative bacteria has a thin peptidoglycan cell wall and a tough outer membrane. The outer membrane does not allow the lysozyme to come in contact with the cell wall keeping it intact.
Hence, Gram positive bacteria will be lysed whereas gram negative bacteria will be unaffected by lysozyme treatment.
<span>This is a form
of asexual reproduction which the bud can grow at specified parts of the plant.
This is caused by the proliferation of the cytoplasm, allowing it to
replicating its parent organism. It can either exist independently or continue
to be attached to its parent organism.</span>
Centrosome is a structure with the function of main microtubule organizing center (MTOC) of the animal cell. This structure has a key role in mitosis where it acts as a regulator of cell-cycle progression. Centrosome is composed of two centrioles which serve to organize microtubules durind the cell division.
Abiotic factors will Define which organisms are able or not to live in a specified place. The living organisms will constitute the biotic factors, which Define if and how can an organism live in a specified environment. So, the abiotic factors are controlling the biotic factors of an environment.