The initiation of DNA replication occurs in two steps. First, a so-called initiator protein unwinds a short stretch of the DNA double helix. Then, a protein known as helicase attaches to and breaks apart the hydrogen bonds between the bases on the DNA strands, thereby pulling apart the two strands.
A Personal Issue is an issue that is then of an individual or also in a relationship causing then a potential conflict or concern. If this will not be resolve or if nothing will be done then it can lead to a big problem then.
